E 418 Gellan Gum – Eu Specification

COMMISSION REGULATION (EU) No 231/2012 of 9 March 2012



Gellan gum is a high molecular weight polysaccharide gum produced by a pure culture fermentation of a carbohydrate by strains of Pseudomonas elodea, purified by recovery with propan-2-ol or ethanol, dried, and milled. The high molecular weight polysaccharide is principally composed of a tetrasaccharide repeating unit of one rhamnose, one glucuronic acid, and two glucoses, and substituted with acyl (glyceryl and acetyl) groups as the O-glycosidically linked esters. The glucuronic acid is neutralised to a mixed potassium, sodium, calcium, and magnesium salt

Einecs: 275-117-5

Chemical name:

Chemical formula:

Molecular weight: Approximately 500 000

Assay: Yields, on the dried basis, not less than 3,3 % and not more than 6,8 % of CO 2


An off-white powder


Solubility: Soluble in water, forming a viscous solution. Insoluble in ethanol


Loss on drying: Not more than 15 % after drying (105 °C, 2,5 hours)

Nitrogen: Not more than 3 %

Propan-2-ol: Not more than 750 mg/kg

Arsenic: Not more than 3 mg/kg

Lead: Not more than 2 mg/kg

Mercury: Not more than 1 mg/kg

Cadmium: Not more than 1 mg/kg

Microbiological criteria

Total plate count: Not more than 10 000 colonies per gram

Yeast and moulds: Not more than 400 colonies per gram

Escherichia coli:  Negative in 5 g

Salmonella spp.:  Negative in 10 g