Prepared at the 69th JECFA (2008), published in FAO JECFA Monographs 5 (2008), superseding tentative specifications prepared at the 67th JECFA (2006) and published in FAO JECFA Monographs 3 (2006). An ADI "not specified" was established at the 19th JECFA (1975).
Gum cyamopsis, guar flour; INS No. 412
Primarily the ground endosperm of the seeds from Cyamopsis tetragonolobus (L.) Taub. (Fam. Leguminosae) mainly consisting of high molecular weight (50,000-8,000,000) polysaccharides composed of galactomannans; the mannose:galactose ratio is about 2:1. The seeds are crushed to eliminate the germ, the endosperm is dehusked, milled and screened to obtain the ground endosperm (native guar gum). The gum may be washed with ethanol or isopropanol to control the microbiological load (washed guar gum).
White to yellowish-white, nearly odourless, free-flowing powder
Thickener, stabilizer, emulsifier
|Insoluble in ethanol|
Add small amounts of sodium borate TS to an aqueous dispersion of the sample; a gel is formed.
Transfer 2 g of the sample into a 400-ml beaker and moisten thoroughly with about 4 ml of isopropanol. Add, with vigorous stirring, 200 ml of water and continue the stirring until the gum is completely and uniformly dispersed. An opalescent, viscous solution is formed. Transfer 100 ml of this solution into another 400-ml beaker, heat the mixture in a boiling water bath for about 10 min and cool to room temperature. There is no substantial increase in viscosity (differentiating guar gum from carob bean gum).
Gum constituents (Vol.4)
Proceed as directed under Gum Constituents Identification using 100 mg of the sample instead of 200 mg and 1 to 10 μl of the hydrolysate instead of 1 to 5 μl. Use galactose and mannose as reference standards. These constituents should be present.
Place some ground sample in an aqueous solution containing 0.5% iodine and 1% potassium iodide on a glass slide and examine under microscope. Guar gum shows close groups of round to pear formed cells, their contents being yellow to brown.
Loss on drying (Vol.4)
Not more than 15.0% (105o, 5 h)
Absent by the following test
Disperse 1 g of the sample in 100 ml of water. The dispersion should remain fluid and not form a gel on standing. Mix 10 ml of dilute hydrochloric acid with the dispersion, and apply one drop of the resulting mixture to turmeric paper. No brownish red colour is formed.
Total ash (Vol.4)
Not more than 1.5% (800°, 3-4 h)
Acid-insoluble matter (Vol.4)
Not more than 7.0%
Not more than 10.0%
Proceed as directed under Nitrogen Determination (Kjeldahl Method) in Volume 4 (under “General Methods, Inorganic components”). The percentage of nitrogen determined multiplied by 6.25 gives the percentage of protein in the sample.
Not more than 1% of ethanol or isopropanol, singly or in combination
See description under TESTS
Not more than 2 mg/kg
Determine using an AAS/ICP-AES technique appropriate to the specified level. The selection of sample size and method of sample preparation may be based on the principles of the methods described in Volume 4 (under “General Methods, Metallic Impurities”).
Microbiological criteria (Vol.4)
Initially prepare a 10-1 dilution by adding a 50 g sample to 450 ml of Butterfield's phosphate-buffered dilution water and homogenizing the mixture in a high-speed blender.
Total (aerobic) plate count : Not more than 5,000 CFU/g
E. coli: Negative in 1g
Salmonella: Negative in 25g
Yeasts and moulds: Not more than 500 CFU/g
Determine by gas chromatography in Volume 4 (under “AnalyticalTechniques, Chromatography”).
Column: 25% Diphenyl-75% dimethylpolysiloxane (60 m x 0.25 mm i.d., 0.25 μm film) [Aquatic-2 (GL-Sciences Inc.) or equivalent]
Solvent standard solution: Transfer 100 mg each of chromatography grade ethanol and isopropanol into a 100-ml volumetric flask containing about 90 ml water and dilute to 100 ml with water. TBA standard solution: Transfer 100 mg of chromatography grade tertiary-butyl alcohol (TBA) into a 100-ml volumetric flask containing about 90 ml water and dilute to 100 ml with water. Mixed standard solutions: Transfer 1, 2, 3, 4 and 5 ml of Solvent
Disperse 1 ml of a suitable antifoam emulsion, such as Dow-Corning G-10 or equivalent, in 200 ml of water contained in a 1000-ml 24/40 round-bottom distilling flask. Add about 4 g of the sample, accurately weighed, and shake for 1 h on a wrist-action mechanical shaker. Connect the flask to a fractionating column, and distil about 95 ml, adjusting the heat so that foam does not enter the column. Add 4 ml of TBA standard solution to the
Inject 1 μl of each Mixed standard solution into the chromatograph. Measure the peak areas for each solvent and TBA. Construct the standard curves by plotting the ratios of the peak areas of each of the solvents/TBA against the concentrations of each solvent (mg/ml) in the Mixed standard solutions.
Inject 1 μl of the Sample solution into the chromatograph. Measure the peak areas for each solvent and TBA. Calculate the ratios of the peak areas of each solvent/TBA, and obtain the concentration of each solvent from the standard curves.
Calculate the percentage of each solvent from:
% Solvent = (C x 100/W x 1000) x 100