Prepared at the 69th JECFA (2008), published in FAO JECFA Monographs 5 (2008), superseding tentative specifications prepared at the 67th JECFA (2006) and published in FAO JECFA Monographs 3 (2006). An ADI "not specified" was established at the 25th JECFA (1981).
Locust bean gum, INS No. 410
Primarily the ground endosperm of the seeds from Ceratonia siliqua (L.) Taub. (Fam. Leguminosae) mainly consisting of high molecular weight
White to yellowish white, nearly odourless powder
Thickener, stabilizer, emulsifier, gelling agent
Insoluble in ethanol
Add small amounts of sodium borate TS to an aqueous dispersion of the sample; a gel is formed.
Transfer 2 g of the sample into a 400-ml beaker and moisten thoroughly with about 4 ml of isopropanol. Add, with vigorous stirring, 200 ml of water and continue the stirring until the gum is completely and uniformly dispersed. An opalescent, slightly viscous solution is formed. Transfer 100 ml of this solution into another 400-ml beaker. Heat the mixture in a boiling water bath for about 10 min and cool to room temperature. There is an appreciable increase in viscosity (differentiating carob bean gum from guar gum).
Gum constituents (Vol.4)
Proceed as directed under Gum Constituents Identification (FNP 5) using 100 mg of the sample instead of 200 mg and 1 - 10 µl of the hydrolysate instead of 1 - 5 µl. Use galactose and mannose as reference standards. These constituents should be present.
Disperse a sample of the gum in an aqueous solution containing 0.5% iodine and 1% potassium iodide on a glass slide and examine under microscope. Carob bean gum contains long stretched tubiform cells, separated or slightly interspaced. Their brown contents are much less regularly formed than in Guar gum.
Loss on drying (Vol.4)
Not more than 14.0% (105º, 5 h)
Total ash (Vol.4)
Not more than 1.2% (800º, 3-4 h)
Acid-insoluble matter (Vol.4)
Not more than 4.0%
Not more than 7.0%
Proceed as directed under Nitrogen Determination (Kjeldahl Method) in Volume 4 (under “General Methods, Inorganic components”). The percentage of nitrogen determined multiplied by 6.25 gives the percentage of protein in the sample.
Not detectable by the following method: To a 1 in 10 solution of the sample add a few drops of iodine TS. No blue colour is produced
Not more than 1% of ethanol or isopropanol, singly or in combination
See description under TEST
Not more than 2 mg/kg
Determine using an AAS/ICP-AES technique appropriate to the specified level. The selection of sample size and method of sample preparation may be
Microbiological criteria (Vol.4)
Initially prepare a 10-1 dilution by adding a 50 g sample to 450 ml of Butterfield's phosphate-buffered dilution water and homogenizing the mixture
Total (aerobic) plate count: Not more than 5,000 CFU/g
Determine by gas chromatography in Volume 4 (under “AnalyticalTechniques, Chromatography”).
Column: 25% Diphenyl-75% dimethylpolysiloxane (60 m x 0.25 mm i.d., 0.25 μm film) [Aquatic-2 (GL-Sciences Inc.) or equivalent]
Solvent standard solution: Transfer 100 mg
Disperse 1 ml of a suitable antifoam emulsion,
Inject 1 μl of the Sample
Calculate the percentage of each solvent from:
% Solvent = (C x 100/W x 1000) x 100