|Prepared at the 49th JECFA (1997) ,published in FNP 52 Add 5 (1997) superseding specifications prepared at the 44th JECFA (1995), published in FNP 52 Add 3 (1995). Metals and arsenic specifications revised at the 49th JECFA (1997). An ADI 'not specified' was established at the 39th JECFA (1992)|
INS No. 400
Alginic acid is a naturally occurring hydrophilic colloidal polysaccharide obtained from the various species of brown seaweed (Phaeophyceae). It is a linear copolymer consisting mainly of residues of ß-1,4-linked D-mannuronic acid and a-1,4-linked L-glucuronic acid. These monomers are often arranged in homopolymeric blocks separated by regions approximating an alternating sequence of the two acid monomers.
The number and sequence of the Mannuronate and Glucuronate residues shown above vary in the naturally occurring alginate. The associated water molecules are not shown.
Structural unit: 176.13 (theoretical), 200 (actual average)
Macromolecule : 10,000 - 600,000 (typical average)
Yields, on the dried basis not less than 20.0% and not more than 23.0% of carbon dioxide (CO2), equivalent to not less than 91.0% and not more than 104.5% of alginic acid (C6H8O6)n .
White to yellowish brown filamentous, grainy, granular or powdered forms
Stabilizer, thickener, gelling agent, emulsifier
Insoluble in water and organic solvents; dissolves slowly in solutions of sodium carbonate, sodium hydroxide and trisodium phosphate
2.0-3.5 (0.3 in 10 suspension)
Precipitate formation with with ammonium sulfate
To a 0.5% solution of the sample in sodium hydroxide TS add one-half of its volume of a saturated solution of ammonium sulfate. No precipitate is formed. This test distinguishes alginic acid from agar, sodium carboxymethyl cellulose, carrageenan, de-esterified pectin, gelatin, carob bean gum, methyl cellulose and starch.
Test for alginate
Dissolve as completely as possible 0.1 g of sample by shaking with 0.15 ml of 0.1 N sodium hydroxide and add 1 ml of acid ferric sulfate TS. Within 5 min, a cherry-red colour develops that finally becomes deep purple.
Loss on drying
Not more than 15% (105o, 4 h)
Not more than 8% on the dried basis
Sodium hydroxide insoluble matter
Not more than 2% on the dried basis
Weigh accurately about 1 g of the sample and dissolve in 100 ml of sodium hydroxide TS, centrifuge and decant. Wash the residue five times with water by mixing, centrifuging and decanting. Transfer the residue by means of water to a tared fine glass filter, dry for 1 h at 105o, cool and weigh. Calculate as percentage of the dry weight.
Not more than 3 mg/kg (Method II)
Not more than 5 mg/kg
Determine using an atomic absorption technique appropriate to the specified level. The selection of sample size and method of sample preparation may be based on the principles of the method described in Volume 4, “Instrumental Methods.”
Total plate count: Not more than 5,000 colonies per gram.
Initially prepare a 10-1 dilution by adding a 50 g sample to 450 ml of Butterfield's phosphate buffered dilution water and homogenizing in a high speed blender.
Yeasts and moulds: Not more than 500 colonies per gram
Coliforms: Negative by test
Salmonella: Negative by test
METHOD OF ASSAY
Proceed as directed under Carbon Dioxide Determination by Decarboxylation in the General Methods. Each ml of 0.25 N sodium hydroxide consumed is equivalent to 5.5 mg of carbon dioxide (CO2) or 25 mg of alginic acid (equivalent weight 200).